The soil fertility concept based on chemical approach has been replaced by the conservation agriculture focus, in which the optimization of production systems promotes integral soil fertility – biological, physical, and chemical. Under such approach, the soil structure assumes relevant role as a result of the quantity and quality of plant mass generated, as well as the frequency of addition to the soil of this plant mass generated by the production model. Under no tillage system, in the evaluation of the integral soil fertility, the challenge is the soil heterogeneity as a result of the limited soil mobilization in the seeding row, of the deposition of limestone and fertilizer on the soil surface or on the surface layer, and of the recurrent seeding rows from harvest to harvest, that affect soil sampling and, mainly the interpretation of soil analytical results. The objective of this study was to evaluate the effect of different plant species, on the heterogeneity of a soil managed under no tillage system, throughout analysis and interpretation of soil physical characteristics considered physical fertility indicators. In a demonstration plot, characterized by harvesting-seeding process structured in four randomized split block of strip crop, the physical soil characteristics were determined in undisturbed soil samples collected in the seeding rows of brachiaria (Brachiaria brizantha), corn (Zea mays (L.)) and soybean (Glycine max (L.)), and in between seeding rows of soybean in the 0-5, 5-20, and 20-30 cm deep. It was concluded that: the crops of the production model studied generate heterogeneity in soil physical characteristics, mainly in the surface layer; the bulk density, total soil porosity and soil macro porosity are sensitive attributes to indicate soil heterogeneity generated by the different crops of the production model; and the brachiaria and corn induce improvements in soil physical fertility by reducing soil bulk density, and increasing total soil porosity, and soil macro porosity in the surface layer.